[Tortwag]

OH MY GOD. That piano. I had forgotten about it. GOSH, King Boo really had some of the creppiest things in his manor.

[Sable-Xeno]

Generic response, but...the Moon from Majora's Mask.

..Oh. And like everyone's been saying, yes, the Piano as well.

[Dish]

...all of Luigi's Mansion. 

[Artsy]

The Golden Monkey in the Golden Compass game. Scary in the movie, scarier in the game.

Also, I don't know if these really scare me, but the darkened rooms in Goof Troop make me really nervous, because I can never tell where the enemies are and I waste a bunch of lives 

And last but not least, the VENUS FLY TRAPS IN LUNATEA'S VEIL. I DON'T KNOW WHY BUT THEY'RE REALLY SCARY

[SyraxAxoJaxco]

....Plants Vs. Zombies, the gameover scream....
....i was such stupid....

That piano (i played it in my DS), my reaction was, "W-whoa!"
Not too scary. :3

[AlphaRed9X]

When I was little, I was pretty much traumatized by the movie "Jaws". So for a while, I was scared of water levels in general, because I'd always assume that a shark would chase after me...

[VikVanHazel]

This.

To be honest, the music on this map freaked me out more than John Romero's head back in the day. The music actually sounds the same when you play it backwards!

[Randomizer]

I should probably add this here as well.

[Enchlore]

Game Over screens. Notably the ones from SNES Starfox, Smash Bros. 64, and Banjo-Kazooie. These ones scared the ever-loving cr*p outta me growing up as a backseat gamer.

I was pretty afraid of game over screens too, for some reason. I think it was Mega Man Legends that had this game over sequence that was a pitch black screen and there was an ominous sound effect leading up to a big red 'Game Over'. Not sure why it made me so afraid as to run away from my room.

Also when a glitch happens that makes the game freeze with that bleeping sound. That scared me so much. I used to completely stop playing certain games just because they froze that way once.

[Braixen]

I don't really know if this counts but when I was little I put a .EXE file (That was a game) into the audio player when that still worked thinking it would play the game's soundtrack sort of like a PS1 disk and instead got the pleasant surprise of

AND THREW MY HUGE BRICK OF A WINDOWS XP LAPTOP THINKING IT WAS POSSESSED